CD16 anticorps

N° du produit ABIN120566

L’anticorps Souris Monoclonal anti-CD16 a été validé pour FACS, IP, WB et Func. Il convient pour détecter CD16 dans des échantillons de Humain.

Aperçu rapide pour CD16 anticorps (ABIN120566)

Antigène: CD16

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD16 est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Functional Studies (Func)

Clone: MEM-154

Détail du produit anti-CD16 anticorps

Specificité: The antibody reacts with with the epitope on CD16 antigen that residing in proximity to FG loop (probably BC or C'E loop). The antibody MEM-154 recognizes a polymorphism of CD16a (NK cells form), it reacts well with granulocyte form (CD16b) of all subjects.

Purification: Affinity Chromatography on Protein-A

Immunogène: Human granulocytes

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry: Recommended dilution: 5-10 m g/mL. Positive control: PBL (peripheral blood lymphocytes) Immunoprecipitation. Western Blotting (non reducing conditions). Functional Application (The antibody blocks binding of human IgG to FcgammaRIII).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: TBS, pH ~8.0 containing 15 mM Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2-8 °C for up to one month or at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Date de péremption: 12 months

Détails sur CD16

Antigène: CD16

Autre désignation: CD16

Sujet: CD16 (FcgRIII) is a 50-80 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgRIII is expressed in two forms FcgRIII-A and -B. FcgRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FceRI-g subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgRIII-A is associated, moreover, with FceRI-b subunit. Besides IgG, FceRI-A can be triggered also by oligomeric IgE. FcgRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.Synonyms: CD16A, FCG3, FCGR3, FCGR3A, Fc-gamma RIII, Fc-gamma RIII-alpha, Fc-gamma RIIIa, FcR-10, FcRIII, FcRIIIa, IGFR3, IgG Fc receptor III-2, Low affinity immunoglobulin gamma Fc region receptor III-A

ID gène: 2214

UniProt: P08637

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process